Ccna Collaboration Cicd 210-060 Official Cert Guide Pdf BEST Download – 📤

Ccna Collaboration Cicd 210-060 Official Cert Guide Pdf BEST Download – 📤

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CCNA Collaboration CICD 210-060 Official Cert Guide is licensed by. CCNP Collaboration CICD 210-060 Official Cert Guide is licensed by, and published by Cisco Systems, Inc.
CCNA Collaboration CICD 210-060 Official Cert Guide This workshop is not. Published by, and published by Cisco Systems, Inc.Esterase gene cloned from Streptomyces sp. ZJ07-6: cloning, sequence and analysis of the active site.
The esterase gene (EST2) of Streptomyces sp. ZJ07-6, which encodes the positive and weak acid esterase II of this strain, was cloned by screening a genomic library with an oligonucleotide probe with DNA sequence homology to the active site of Est2. The nucleotide sequence of Est2 was derived from the DNA sequence of a subclone (Est2c) determined by sequencing of genomic clones and EST2c contains an open reading frame of 1,120 bp encoding a protein of 373 amino acids with a calculated molecular mass of 41.9 kDa. A peptide sequence homology search revealed that the amino acid sequence of Est2 is homologous to other amino acid sequences of type I alpha-sulfatase family esterases. The EST2 gene was expressed in Escherichia coli and the expressed protein was purified and shown to be active. Homology modeling of the deduced tertiary structure of the active site region of Est2 showed that it is composed of five loops, the N-terminal beta/alpha barrel, the C-terminal alpha/beta barrel, the proline bridge and the catalytic center loops. The modeled structure of Est2 is significantly similar to those of other type I alpha-sulfatases and has a negative charge in the catalytic center loop of Est2. Based on these results, it was concluded that Est2 is a member of the esterase family and the active site contains five loops named A-F loops. UNPUBLISHED

UNITED STATES COURT OF APPEALS
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